Laws enacted by the U.S. Department of Health and Human Services (DHHS) to safeguard subjects from dangers associated with research projects that any federal department or agency participates in.
What did 45 CFR 46 do?Laws enacted by the U.S. Department of Health and Human Services (DHHS) to safeguard subjects from dangers associated with research projects that any federal department or agency participates in.Also known as Protection of Human Subjects, 45 CFR Part 46, and human participant protection regulations.45 CFR 46 of the Federal Rules.In order to safeguard study subjects, investigators and IRBs must adhere to the "Protection of Human Subjects" regulation, sometimes known as the "Common Rule".US Public Health Service Guidelines (45 CFR 46 raised to regulatory status the US Public Health Service policy of 1966 "Clinical research and investigation involving human beings".)To learn more about DHHS refer
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which of the following is not a characteristic shared by all chordates, at least in some part of their lifecycle?
Four-chambered heart is a characterastic which is not shared by all chordates in some of their lifecycle.
The left and right halves of the four-chambered heart are fully divided by septa. This stops the mixing of oxygenated and deoxygenated blood. All regions of the body can now receive oxygenated blood very effectively. This is helpful for species with high energy requirements, such birds and mammals.
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If the substrate for an enzyme catalyzed reaction contained a negative charge, which of the following amino acids would most likely be present int eh active site to provide electrostatic destabilization of the ES complex? Why? A. Val B. Asp C. Arg D. Ser E. Gln
Asp amino acids would probably be present at the active site to provide electrostatic destabilization of the ES complex if the substrate for an enzyme-catalyzed reaction carried a negative charge.
The answer to the question is which of the following is not likely to contribute, therefore the last side of an enzyme does not contribute to replacement specificity. Allosteric side cannot bind because the lasteric side cannot bind with the replacement. Allosteric side of an enzyme interacts with the modulator molecule since it is a regulatory side of the enzyme. The compounds known as catalysts speed up chemical reactions without contributing to them. The biological reactions that take place in living organisms are catalysed by enzymes. The bulk of enzymes are made up of proteins, while some ribonucleic acid (RNA) molecules also perform this role.
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This diagram orders biological systems from least complex (at the beginning)
to most complex at the end):
Population - Community - Ecosystem - Biome
Which systems include abiotic factors?
O A. Population and community only
• B. Community and ecosystem only
• C. Community, ecosystem, and biome only
O D. Ecosystem and biome only
Community, ecosystem, and biome only include abiotic factors.
What is an example of an abiotic factor in an ecosystem? An abiotic factor in an ecosystem is a non-living element that can affect the environment and the organisms within it. Examples of abiotic factors include climate, soil, air temperature, water, sunlight, and other physical and chemical elements of the environment.Climate is one of the most important abiotic factors in an ecosystem, as it can affect the ability of organisms to survive. For example, high temperatures may limit the range of certain species, while extreme cold can kill off entire populations.Soil is also an abiotic factor that can affect an ecosystem. It can provide nutrients for plants and provide a habitat for microorganisms. Soil composition and texture can also influence the types of plants that can grow and the number of animals that can inhabit an area.Air temperature is another important abiotic factor, as it can influence the activity of organisms, such as the speed at which plants and animals grow and reproduce.Water is also an important abiotic factor, as it is necessary for most organisms to survive. It also influences the type of organisms that can inhabit an area, as some species may require certain levels of water and salinity.Sunlight is a major abiotic factor, as it provides energy and warmth to plants and animals, and is necessary for photosynthesis to occur.All of these abiotic factors interact with one another and with the biotic factors in an ecosystem, such as plants and animals, to create a balanced and healthy environment. Without these abiotic factors, ecosystems would not be able to function.
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I need help with this:(
Answer:
Water temperature is a key factor affecting phytoplankton bloom dynamics in shallow productive coastal waters and could become crucial with future global warming by modifying bloom phenology and changing phytoplankton community structure, in turn affecting the entire food web and ecosystem services.
Which of the following contains all the possible phenotypes that could be the result of parents who are type O and type A:
Please select the single best answer
Type A or type O only
Type A only
Type O only
All possible blood types
All possible blood types. The possible blood types that could result from parents who are type O and type A are A, O, AB, and AO.
What is blood types?Blood types refer to the classification of blood based on the presence or absence of certain antigens on the surface of red blood cells. Blood types are determined by the ABO blood group system, which classifies blood into four major types: A, B, AB, and O. Each of these types is further divided into positive or negative subtypes, depending on whether or not the Rh factor is present. Blood transfusions are only compatible between certain types, making it important to know a person's blood type before donating or receiving blood. In addition to the ABO system, there are other rarer blood types, such as the Kell and Duffy systems, which are also important to consider in transfusions.
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pick one chain from your food web and create an explanation of the energy flow and how energy is being used and lost in the ecosystem: talk about photosynthesis, fermentation and cellular respiration
One example of a food chain can be grass>grasshopper>frog>snake>eagle. In this chain, the grass is the producer which generates energy while the eagle is the apex predator.
In the given food chain, the green plants(grass in this case), use photosynthesis to extract energy from sunlight, and only 10% of solar energy is converted to chemical energy and the remaining 90% is lost. Through each subsequent chain, only 10% of energy is passed on, and the remaining 90% is lost. The animals consume food and then subject it to cellular respiration where the carbohydrates primarily glucose are broken down to release energy in the presence of oxygen. When the glucose breakdown takes place in the absence of oxygen, then the process is called fermentation. Both these methods are used to generate Adenosine triphosphate molecules which serve as energy currency.
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which of the following is not a way in which you can address the physical effects of speech anxiety?
Crying is not one way to address the physical effects of speech anxiety. Therefore, option b is a relevant answer.
Speech anxiety can range from a mild case of the "nerves" to virtually paralyzing fear. Anxiety is frequently manifested as a fear of public speaking. It might be anything from mild uneasiness to paralyzing anxiety and panic. Many persons who suffer from this fear either completely avoid public speaking situations or put up with them by trembling their hands and voice.
Shaking, sweating, quick heartbeat, stomach butterflies, dry mouth, and squeaky voice are some of the most typical signs of speech anxiety. Even though it is frequently hard to eradicate speech anxiety, there are several strategies to manage it and even use it to your benefit. By lowering muscle tension and general mental distress, progressive muscle relaxation aids in breaking the cycle of anxiety. Another way is to stand in a power pose which helps to feel stronger, less stressed, more confident, and less anxious. Therefore, option b is correct because crying helps to reduce the emotional effect of speech anxiety, not the physical effect.
The complete question is -
which of the following is not a way in which you can address the physical effects of speech anxiety?
a) Repeat tensing and relaxing different muscles
b) Cry in private before the speech
c) Yell as loud as you can to release energy
d) Stand in a power pose in private before the speech
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functions of the oral cavity include sensory analysis of food, mechanical processing, and lubrication
a. true
b. false
True functions of the oral cavity include sensory analysis of food, mechanical processing, and lubrication
What is oral cavity ?An oval-shaped aperture in the skull is the mouth, often known as the oral cavity. Starting at the lips and moving down to the throat. Breathing, speaking, and food digestion are just a few of the body processes that depend on it. Tissues are pink, wet, odourless, and painless in a healthy mouth.
Two areas of the oral cavity can be distinguished:
(1) the oral vestibule, which is the area medial to the teeth
(2) the oral cavity proper, which is the area around the teeth.
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a deer waits motionlessly, hidden in the thicket as a band of hunters approaches. as the hunters get closer, their dogs bark, picking up the scent of their prey. in a futile effort to escape, the deer bolts. which of the following most accurately describes the nervous system of the hunted deer at this point? group of answer choices
The appropriate response is (A) Sensory: detects changes in the environment and transmits information to the brain and spinal cord.
Which of the following statements best sums up how the nervous system works? The appropriate response is (A) Sensory: detects changes in the environment and transmits information to the brain and spinal cord.The nervous system's main job is to gather a wide variety of sensory data, analyze, interpret, and integrate that data, and launch the proper bodily reactions as a result.The central organizing mechanism of the organism is the neurological system.Each and every experience, thought, and deed has a reflexive effect.The central nervous system (CNS) and the peripheral nervous system are the two main divisions used to categorize the structures of the nervous system (PNS). Blood flow to the body and the nervous system's management of blood pressure and heart rate.Hormones produced by the endocrine system control neuronal activity.To learn more about nervous system refer
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The primary structure of a protein: is determined using NMR methods: can be determined as long as the peptide is not broken or denatured. is determined by cleaving the protein into smaller peptides. is its sequence of amino acids is determined by combining overlapping peptide segments to obtain the sequence of the entire
The correct statements are - is its amino acid sequence
The size of the protein is determined by cleaving it into smaller peptides.
The sequence of the entire protein is determined by combining overlapping peptide segments.
The primary structure of proteins is the basic structure, which is only a sequence of amino acids.Because the primary structure of the protein has not been denatured, this is the incorrect option.Because any large protein is made up of primary protein, cleavage of protein results in primary structure, which is then determined. As a result, it is correct.We can obtain the entire protein by arranging the primary structure of amino acids in 3D or 2D. As a result, it is also correct.
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which of the following cell types, if absent, would result in the lack of all humoral immune responses?
B Lymphocytes if absent, would result in the lack of all humoral immune responses.
Antibody-producing white blood cells. Immune system components called B lymphocytes are created in the bone marrow from stem cells. called a B cell as well.
Known also as B cells, B lymphocytes produce an antibody, which is a specific kind of protein. To neutralize pathogens or foreign materials like poisons, these antibodies bind to and bind to them. In order to stop a virus from infecting a healthy cell, for instance, an antibody can bind to the virus.
The fundamental processes of producing antibodies and cell-mediated immune responses, respectively, were shown by studies conducted in the 1960s and 1970s to be predominantly carried out by B and T cells.
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Asexually reproducing organisms produce offspring that are genetically identical to each other and to the parents. What type of cell division are the offspring a product of?
A few random individuals colonize an area in which no individuals of their species lives. The allele frequencies of the founding population may not represent the allele frequencies of the mainland population. This form of genetic drift is called:
the founder effect
natural selection
artificial selection
the bottleneck effect
Answer:
The founder effect
Which statement best describes a relationship between activation, energy and rate of reaction
The activation energy of a chemical reaction is closely related to its rate such that the higher the activation energy, the slower the chemical reaction. So, the correct option is (B).
What is Activation energy?Activation energy is defined as the minimum amount of energy that must be provided for compounds to result in a chemical reaction which is measured in joules per mole, kilojoules per mole or kilocalories per mole.
It is the minimum amount of energy required for a chemical reaction to occur. It is defined as the difference between the energies of the reactants and the energy greatest along the reaction progress. The rate of reaction is directly proportional to the activation energy.
Thus, the activation energy of a chemical reaction is closely related to its rate such that the higher the activation energy, the slower the chemical reaction. So, the correct option is (B).
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Your question is incomplete, most probably the complete question is :
Which statement best describes the relationship between activation energy and rate of reaction?
Increasing the activation energy can increase the rate of a reaction.Reducing the activation energy can increase the rate of a reaction.Increasing the reaction energy does not alter the rate of reaction.Reducing the activation energy can decrease the rate of a reaction.Which of the following must happen, ALONG with listing a species in accordance with the Endangered Species Act, to improve a particular species?
Group of answer choices
a there is international cooperation
b it is bred in captivity
c its habitat is preserved
d its genes are preserved
answer asap
Along with listing a species in accordance with the Endangered Species Act, c its habitat is preserved must happen to improve a particular species.
What is the Endangered Species Act?The Endangered Species Act is a law generated in order to protect species that are threatened or endangered due to human activities, including habitat destruction, pollution, and over-exploitation, which is achieved by using scientific and commercial data to make their decisions.
Therefore, with this data, we can see that Endangered Species can be very useful in order to protect species from extinction.
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disruption of the normal microbiota can result in infections caused by which of the following microbes?
Clostridium difficile. Broad-spectrum antibiotic treatment over an extended period of time can alter the typical microbe in the human intestines.
Irritable bowel syndrome (IBS) and inflammatory bowel disease (IBD), as well as more widespread systemic manifestations of disease including obesity, type 2 diabetes, and atopy, have all been associated with an imbalance of the normal gut microbe. When these antibiotics eliminate the healthy gut microbiota, Clostridium difficile populations can rise, leading to the potentially lethal illness known as pseudomembranous colitis. A variety of disorders, including obesity, type 2 diabetes, hepatic steatosis, inflammatory bowel diseases (IBDs), and various cancer types are now known to be correlated with abnormalities in the gut microbiota. As a result, it is possible that different metabolic pathways involved in immunity, energy, lipid, and glucose metabolism are impacted.
The complete question is:
Disruption of the normal microbiota can result in infections caused by which of the following microbes?
Candida albicans
Mycobacterium
Clostridium difficile
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When a solution has carbohydrates in it, the copper in Benedict's gets reduced and the solution stays blue.
a. true
b. false
Answer:
This is TRUE
Explanation:
One of the main principles of the scientific method is Replication. Benedict's test not only detects reducing carbohydrates as a yes/no answer, but also how much reducing sugar is present in the solution.
identify the species that behaves as the oxidizing agent in the following reaction. zn 2 agno3 -> zn(no3) 2 2ag
The species that behaves as the oxidizing agent in the given reaction is AgNO3.
What is oxidizing agent?
An oxidizing agent is a molecule or substance that has the ability to oxidize other molecules or substances.
Oxidation is a chemical reaction in which electrons are transferred from one molecule to another, resulting in the formation of a new compound.
Oxidizing agents act as electron acceptors, and usually contain oxygen, halogens, or other elements that can easily gain electrons. Oxidizing agents are used in a variety of chemical processes, including the production of fuels and pharmaceuticals, and in industrial processes such as metal etching and water treatment.
Therefore, The species that behaves as the oxidizing agent in the given reaction is AgNO3.
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because the eleventh and twelfth pairs of ribs do not attach anteriorly, they are called floating ribs.
a. true
b. false
The eleventh & twelfth are free and lack any anterior articulations. The eleventh & twelfth thoracic ribs serve as their only posterior attachment points. These ribs are known as floating ribs since they don't appear to be fastened anteriorly.
The lower two sets of ribs of the rib cage do not connect to the sternum. The 11th and 12th sets of ribs are floating ribs. The anterior border is free on ribs 11 and 12, which are referred to as floating ribs.The eleventh & twelfth are free and lack any anterior articulations. The eleventh & twelfth thoracic ribs serve as their only posterior attachment points. These ribs are known as floating ribs since they don't appear to be fastened anteriorly. 2 The term "intercostal gaps" refers to the area between the ribs. The three portions of a rib—the posterior vertebral, anterior or sternal, as well as the shaft of the rib—are made of cancellous bone tissue.
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endor and special triple resonance spectroscopy of photoaccumulated semiquinone electron acceptors in the reaction centers of green sulfur bacteria and heliobacteria
The spectroscopy of photo accumulated semiquinone electron acceptors in the reaction centers of green sulfur bacteria and heliobacteria can be studied using proton-detected Over Hauser and special triple resonance spectroscopies.
What is mean by triple resonance spectroscopy?Triple resonance spectroscopy is a powerful tool for studying the structure and dynamics of photo accumulated semiquinone electron acceptors in the reaction centers of green sulfur bacteria and heliobacteria. This technique combines the advantages of two-dimensional (2D) nuclear magnetic resonance (NMR) spectroscopy with those of electron paramagnetic resonance (EPR). It allows us to measure the coupling between nuclear spins, the electron spin, and the magnetic field in the sample, which can then be used to study the structure, dynamics, and energetics of the semiquinone. This information is essential for understanding the role of semiquinone in the electron transfer reactions in these photosynthetic organisms. Furthermore, triple resonance spectroscopy can be used to study the effects of light and temperature on the semiquinone and its environment, as well as its interactions with other molecules.To learn more about spectroscopy refer to:
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. Is the frilled lizard unicellular or multicellular? Use evidence to explain your answer.
The frilled lizard is multicellular organisms.
What do you mean by multicellular organisms?Multicellular organisms are those composed by multiple cells. They are classified in 13 major groups of terrestrial living beings, including animals, plants, fungi, ciliates, algae, and foraminifera.
As well as humans, plants, animals and some fungi and algae are multicellular. A multicellular organism is always eukaryote and so has cell nuclei. Humans are also multicellular.
Simple multicellular organisms, composed of multiple similar cells, occur widely within the eukaryotic tree of life, and a few of these evolved into more complex multicellular organisms characterized by differentiated tissues and organs.
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Name 2 differences between genetic disorders, viral infections, bacterial infections, and cancer. I need 2 differences between all 4 please. Thank you in advance!
The differences between genetic disorders, viral infections, bacterial infections, and cancer are that the genetic disorder is inherited, while the viral infection can be transferred from one person to another but not in the case of the bacterial infection. Cancer is caused by abnormal cell division.
What is the significance of the bacterial and viral infections?Genetic disorders can be caused by mutations, but viral infections, bacterial infections, and cancer are caused by external agents like chemical aging or other infections. While the genetic disorder is passed from the parent to the offspring, viral infections, bacterial infections, and cancer can occur at any point in an individual's life and can be influenced by the environment.
Hence, the differences between genetic disorders, viral infections, bacterial infections, and cancer are that the genetic disorder is inherited, while the viral infection can be transferred from one person to another but not in the case of the bacterial infection. Cancer is caused by abnormal cell division.
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A 10 year-old child received one dose of the hepatitis A vaccine at 1 year of age, but never returned for a second dose. Which of the following is true regarding the need for a second dose of the hepatitis A vaccine for this child?
A. No further doses of hepatitis A vaccine are required
B. Wait until the child is 18 years of age and give the second dose if there are other risk factors
C. Give 1 dose now as catch up
D. Restart the series and give 2 doses , 6 months apart
Answer: D
Explanation:
A 5-day-old infant was born with a laryngeal defect. The greater cornu and the inferior part of the hyoid bone were absent at birth. Failure of development of which of the following embryonic structures most likely led to these defects?
A. Maxillary prominence B. Mandibular prominence
C. Second pharyngeal arch
D. Third pharyngeal arch E. Fourth pharyngeal arch
Option C; The failure of the development of second pharyngeal arch leads to laryngeal defect.
The embryonic structure that is most likely responsible for the laryngeal defect described in the 5-day-old infant is the pharyngeal arch. The branchial arches are a series of structures that form in the embryonic stage and give rise to various structures in the head and neck, including the hyoid bone and the larynx. Specifically, the greater cornu and the inferior part of the hyoid bone develop from the second branchial/pharyngeal arch. Therefore, failure of development of the second branchial arch would result in the absence of these structures at birth.
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measurements of oxygen electroadsorption energies and oxygen evolution reaction on ruo2(110): a discussion of the sabatier principle and its role in electrocatalysi
The Sabatier Principle states that the energy required to electroadsorb oxygen onto the surface of RuO2(110) must be greater than the energy released from the oxygen evolution reaction. This principle plays an important role in electrocatalysis, as it helps to ensure that the reaction is thermodynamically favorable.
What are the challenges associated with measuring oxygen electroadsorption energies and oxygen evolution reaction on RuO2(110)? Measuring oxygen electroadsorption energies and oxygen evolution reaction on RuO2(110) can be challenging due to the complexity of the surface. The electronic structure of RuO2(110) is highly affected by the oxidation state of the surface. Therefore, the energetics of oxygen adsorption and the oxygen evolution reaction on this surface can be difficult to quantify. Additionally, the presence of oxygen vacancies and oxygen interstitials on the surface can result in a non-uniform environment, which can lead to discrepancies in the measurements. Furthermore, the structure of RuO2(110) can become unstable when exposed to high temperatures, making it difficult to accurately measure the energies at elevated temperatures. Finally, the presence of adsorbates on the surface can also affect the electronic structure and energetics, further complicating the measurements.To learn more about measurements of oxygen refer to:
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Review the relationship between genotype and phenotype (see Figures 14.5 and 14.6). Suppose that in a particular pea population, flowers with the white phenotype are favoured by natural selection. Predict what would happen over time to the frequency of the p allele in the population, and explain your reasoning.
Although genotype and phenotype are two distinct ideas, they are still connected.
The physical characteristics of an organism that are the result of the expression of a person's genes are referred to as phenotypes. An individual's phenotype is determined by genotype. An organism's phenotype is made up of all of its observable traits.
Phenotypes and genotypes vary significantly in that the former is inherited from an organism's parents, whilst the latter is not. Although a genotype can impact a phenotype, the opposite is also true.
The traits are the same in homozygous dominant and heterozygous genotypes. What distinguishes genotypes from alleles? Genes come in different forms called alleles, whereas genotypes are the collection of genes that determine a certain feature.
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The speed of sound in steel is greater than it is in air because
steel is
a. more elastic than air
b. less elastic than air
c. more dense than air
d. less dense than air
For a wave of wavelength 4 m and frequency 8 waves/sec.,
a. amplitude = 32 m.
b. amplitude = 0.5 m.
c. speed =32 m/sec.
d. speed = 2 m/sec.
a) The speed of sound in steel is greater than it is in air because
steel is more dense than air. Option C
b) The speed of the wave is 32 m/sec. Option C
What is the speed of a wave?We know that the speed of a wave is the ratio of the distance that have been covered by the wave within a given time interval. We ought to know that the speed of sound in various media do differ. This stems from the fact that density of the media also differ.
We know that;
Wavelength = 4 m
Frequency of the wave = 8 waves/sec.,
Speed of the wave = Wavelength * Frequency
= 4 * 8 = 32 m/s
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watch anne rice's mayfair witches trailer: power | anne rice's mayfair witches video extras premieres sunday, january 8 at 9/8c anne rice's mayfair witches mayfair witches focuses on an intuitive young neurosurgeon (alexandra daddario) who discovers that she is the unlikely heir to a family of witches. coming to amc
Follows a neurosurgeon when she learns she is the improbable heir of a witch lineage. She has to deal with an evil force that has followed her family for many years.
An intuitive young neurosurgeon (Alexandra Daddario) who learns she is the improbable successor to a dynasty of witches will be the main character of the television show. She must battle with a dark ghost (Jack Huston) that has plagued her family for decades while she struggles with her newfound abilities. Rowan seeks out Lestat because she is caught between loving her husband Michael and Lestat. She asks him to turn her into a vampire since she is worn out by life. Despite his pain, Lestat declines because she is the Mayfair family's compass and he cannot be their leader.
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Cytokinesis usually follows mitosis. If a cell completed mitosis but not cytokinesis, the result would be:a) A cell with a single large nucleus.b) A cell with high concentrations of actin and myosin.c) A cell with two abnormally small nuclei.d) A cell with two completely identical nuclei.e) A cell with two non-identical nuclei with half the amount of DNA.
a cell with two nuclei that are completely identical
Mitosis is the process by which a cell replicates and then segregates its chromosomes, producing two identical nuclei in preparation for cell division. Mitosis is commonly followed by the equal division of the cell's content into two daughter cells with identical genomes.
Mitosis is a nuclear division process that occurs in eukaryotic cells when a parent cell divides to produce two identical daughter cells.
Mitosis is a type of cell division in which one cell (the mother) divides to produce two new genetically identical cells (the daughters). Mitosis is the process by which the DNA of the cell's nucleus is divided into two equal sets of chromosomes in the context of the cell cycle.
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match the step of the scientific method with the correct description of the experiment described below. an apple juice manufacturing company wants to increase their production but is limited by the number of apples they are able to harvest each season. one of their growers has proposed the use of a new chemical spray that will increase the yield of apples each tree in an orchard can produce. but the chemical spray is slightly expensive to make. the juice manufacturer wants to test the spray prior to committing to its in their orchards. you are the scientist testing the affects of the chemical. you select two orchards that are 5 miles apart from each other and spray orchard a with the chemical and leave orchard b without the chemical. in the fall, the number of apples harvested from each forest is counted. the orchard a yields an average of 60 apples per tree and the orchard b yields an average of 40 apples per tree. based on your research, the juice manufacturer decides to invest in the chemical spray.
Independent variable: will be variation do not depend on the another. So here it Represents the apple yield per tree.
conclusion: The orchards in which the chemical spray was applied resulted in a greater yield of apples per tree than orchards that were not included. This could lead to an overall greater production rate and an increase in juice profits.
constant: the amount of sunlight each orchard received.
hypothesis: The chemical spray used on the first orchard.
dependent variable: The use if chemical spray X on apple trees results in a greater yield of apples per tree.
The amount of apples that can be harvested each season prevents an apple juice manufacturing company from increasing their production. One of their producers has suggested the usage of a brand-new chemical spray that will boost the amount of apples that each tree in an orchard can produce. However, it costs a little more to produce the chemical spray. Prior to using the spray in their orchards, the juice company wants to test it. Scientist testing the effects of the chemical, you. You pick two orchards that are five miles distant from one another, spray Orchard A with the substance, and leave Orchard B untreated. Each forest harvests an equal number of apples each fall.Both orchards produce an average of 60 apples per tree in orchard A and 40 apples per tree in orchard B. The juice company agrees to purchase the chemical spray after considering your findings.
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