The purpose of using nitrocellulose for a western blot is to a. allow the proteins to be concentrated onto the face of the nitrocellulose where they will be accessible to the primary antibody b. to allow the 4-chloro-1-naphthol to react and be visible c. allow the various proteins to migrate and separate d. bind to the primary antibody

Answers

Answer 1

Answer:

a. allow the proteins to be concentrated onto the face of the nitrocellulose where they will be accessible to the primary antibody

Explanation:


Related Questions

y’all i need help please help

Answers

Answer: It is (D) Skin

Explanation: Skin can absorb materials, it also protects our body, it also regulates our body temp.

Skin does all of those things

No.3 plzz ...?
Any one ​

Answers

2^n=32

n=5

It doesn't really match up with the options so not sure, my best guess would be either choosing 16 or 4. 16 in case the person calculated as 16 times 2 = 32 and 4 bc its the closest.

What are genetic crosses

Answers

Answer:

1:2:1 is the genetic crosses

Both anaerobic and aerobic respiration use what to produce ATP and NADH?

Answers

Answer

glucozo

Explanation:

How Can I Explain Why A Theory Is Also A Hypothesis?

Answers

Answer:

well a hypothesis is literally a scientific assumption or theory used to describe what they think is going to happen during a experiment

Is Brewer's yeast a type of bacteria

Answers

Explanation:

Brewer's yeast is an ingredient used in the production of beer and bread. It is made from Saccharomyces cerevisiae, a one-celled fungus. ... Brewer's yeast is considered a probiotic and is used to aid digestion.

Answer:

Well Brewers Yeast is actually a Probiotic, and well a bacteria as well,

but its a good bacteria.

Explanation:

Explain what will happen to your eyes when..
a) you move in a very bright light.
b) you move in a dim light​

Answers

Answer:

For A your pupils will shrink for B your pupils will dilate.

Answer:

When you look at something that is bright, your pupil may shrink.

When you look at something dim, your pupil may get larger.

Explanation:

I think your pupil may get larger if you look at dim light because your eye may have a hard time seeing what's going on. And the opposite may happen when you look at bright light.

draw a flowchart showcasing the process of scientific learning .also mention a short description of each of the step in the chart. ​

Answers

Answer:

The scientific method is a system of exploring the world around us, asking and answering questions, and making predictions. Scientists use the scientific method because it is objective and based on evidence. A hypothesis is fundamental to the scientific method. A hypothesis can take the form of an explanation or a prediction. There are several ways to break down the steps of the scientific method, but it always involves forming a hypothesis, testing the hypothesis, and determining whether or not the hypothesis is correct. If the hypothesis is rejected, this does not mean the experiment was a failure. In fact, if you proposed a null hypothesis (the easiest to test), rejecting the hypothesis may be sufficient to state the results. Sometimes, if the hypothesis is rejected, you reformulate the hypothesis or discard it and then go back to the experimentation stage.

The process of scientific learning refers to the various steps via which one can examine and study our environment to understand it. Once understanding or know-how is achieved, then technology is said to have advanced further.

It starts with making observations. Now because of the limitation of our senses, it is important not to jump to conclusions. The next step is to form a hypothesis based on the observation madeWhen you have arrived at a hypothesis, you can now carry out a controlled experiment where you collect further data and reach conclusions based on the experimentIf the hypothesis is in line with your observation, you publish resultsIf the hypothesis is null, you also publish results, then review your hypothesis  and repeat the experiment

All the above require consistent documentation at each stage. Please see the attached image for the flowchart.

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In meiosis, sister kinetochores are attached to the same pole of the cell during meiosis I, and sister chromatid cohesion is released during anaphase II. What would be the likely result if sister kinetochores were attached to different poles of the cell during meiosis I and sister chromatid cohesion was released during anaphase I

Answers

Answer:

sister chromatids would migrate to opposite poles in anaphase I

Explanation:

Kinetochores are multiprotein structures that establish the attachment of spindle microtubules to the chromatids during cell division (either mitosis or meiosis). Sister chromatid cohesion at the centromeres refers to the mechanism by which sister chromatids are paired and held together during meiosis I. This process (sister chromatid cohesion) is fundamental to ensure the correct orientation of chromatids on the spindle microtubules and their subsequent segregation in meiosis II. The fusion of sister kinetochores in meiosis I underlies sister chromatid cohesion during this stage of the cell division, thereby their disruption or destruction of sister chromatid cohesion would lead to separation and segregation of sister chromatids to opposite poles of the cell in anaphase I.

Why making anti-viral medicines is harder than making anti-bacterial medicines ?

Also Is Virus a living parasites?​

Answers

Answer:

Making anti-viral drugs is more difficult than making anti-bacterial medicines because viruses have very few biochemical mechanisms of their own. They make use of host machinery which replicates. So it is very much difficult to find the targets where these drugs get interfered by not making any harm to the host. Thus, it is not easy to design an antiviral drug with high safety and effectiveness.

Explanation:

And i would say virus is a parasites but not a living parasite according to md

Answer:

Because antivirals can damage host cells where the viruses reside.

I hope this helps

all viruses are obligate parasites,that is they lack metabolic machinery of their own to generate energy or to synthesize proteins,so they depend on host cells to carry out these vital functions...

Observe and record the seeds, roots, stems, leaves, flowers of mustard and maize plants and the difference in propertiesCheck it out​

Answers

Answer:

Maize is monocot and fibrous roots whereas mustard is dicot and tap roots system.

Explanation:

The main difference between maize plant and mustard plants is that Maize is a monocot plant means only one cotyledon in their seed whereas Mustard are dicot which means having two cotyledons. Maize plant has a fibrous root system whereas mustard plant has tap root system. The leaves pf maize plant have parallel venation while on the other hand, the leaves of mustard having reticulate venation. The seeds of maize is bigger in size as compared mustard seed.

Which is a possible outcome of global warming?
A. Hurricanes and other extreme weather will increase
B. Most crops will grow better in warmer climates
C. Sea levels will drop all around the world
D. Disease will not be able to spread as quickly

Answers

Answer is A While hurricanes are a natural part of our climate system, recent research suggests that there has been an increase in intense hurricane activity in the North Atlantic since the 1970s. In the future, there may not necessarily be more hurricanes, but there will likely be more intense hurricanes that carry higher wind speeds and more precipitation as a result of global warming.

A possible outcome of global warming is that hurricanes and other extreme weather events may increase. Therefore, the correct statement is option A.

What causes an increase in the temperature of Earth?

The gradual rise in the Earth's atmosphere's average temperature that results from the buildup of greenhouse gases, primarily carbon dioxide, released by human activities like the burning of fossil fuels and deforestation is known as global warming.

The Earth's climate and weather patterns may alter as a result of this temperature rise, which might result in more frequent and severe weather events like hurricanes, floods, and droughts.

Due to alterations in precipitation patterns, increased pest pressure, and other factors, it is unlikely that most crops will grow more effectively in warmer climates. Because of the melting of glaciers and ice caps, sea levels are more likely to increase, resulting in floods in low-lying places.

Therefore, a possible outcome of global warming is that hurricanes and other extreme weather events may increase.

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where's ATP produced in a cell and how?​

Answers

The energy released during respiration is stored in the form of compounds called ATP molecules. They are produced by the enzyme ATP synthesis which converts ADP to ATP. It is located in the membrane of the cellular structures called mitochondria.

Answer:

Most of the ATP in cells is produced by the enzyme ATP synthase, which converts ADP and phosphate to ATP. ATP synthase is located in the membrane of cellular structures called mitochondria; in plant cells, the enzyme also is found in chloroplasts.

Explanation:

BRAINLIEST PLZ

Which prediction is true based on this population pyramid?

The population is not growing.


The population is growing slowly.


The population is growing rapidly.


The population is declining.

Answers

I would say the population is growing slowly.

Hopefully that’s right.

1. Assertion: Mother may be a carrier for haemophilia.
Reason: The father is always a carrier for haemophilia.
(a) Both Assertion and Reason are true and the Reason is a correct explanation of the Assertion.
(b) Both Assertion and Reason are true but the Reason is not the correct explanation of the Assertion.
(c) Assertion is true, but the Reason is false.
(d) Assertion is false, but the Reason is true.

Answers

Answer:

c

Explanation:

The assertion is true,but the reason is false because the mother is the carrier.

I hope this helps

Why is it essential for SARS-CoV2 RNA be translated on ribosomes before it can be replicated?

Answers

The SARS-CoV-2 non-structural protein 1 (Nsp1), also referred to as the host shutoff factor, suppresses host innate immune functions. By combining cryo-electron microscopy and biochemistry, we show that SARS-CoV-2 Nsp1 binds to the human 40S subunit in ribosomal complexes, including the 43S pre-initiation complex and the non-translating 80S ribosome. The protein inserts its C-terminal domain into the mRNA channel, where it interferes with mRNA binding. We observe translation inhibition in the presence of Nsp1 in an in vitro translation system and in human cells.

Kể tên các biện pháp đấu tranh sinh học. Cho ví dụ. Biện pháp đấu tranh sinh học có những ưu điểm gì?

Answers

Answer:

means translate in English . if I can help I will.

Why ulva is not placed in kingdom plantae

Answers

Answer:

Because Ulva is an algae. Algae belongs to kingdom Protista.

Explanation:

Ulva is a Macroalgae because it's not microscopic.

Macroalgae is not considered as a plant due to their structure. Macroalgae do not have specialized organs and tissues, and they are not vascularized. They also do not have the capacity to form a structure with flowers, leaves, roots or a stem.  

What type of medication is prescribed for a bacterial infection such as strep throat?
antifungals
antibiotics
antivirals
antiprotozoals

Answers

I believe its Antibiotics

What makes people fight for their humanity in spite of horrendous challenges?

Answers

Answer:

They believe that they should have free will and in their mind they know that if they don`t fight then they can never feel like they belong on this earth and they would do whatever it takes to show people that they have a voice and that it should never be silenced but always be heard. People know even if there are challenges it shows that have got courage to overcome it and get to a better place in life.

Explanation:

Which hair is Is constitute about two third of the hair of women , one tenth of the hair of men, and all of the hair of children?

Answers

Answer:

vellus

Explanation:

The villus hair is also called as the peach fuzz refers to a fine thin and light-colored hair. It covers the majority of body parts such as face, stomach, legs and arms. This hair tends to be very short and has a length of 2mm.

Which population is more difficult to count during a survey of biodiversity?
O A clumped population
O A uniform population
A mobile population
O A small population

Answers

A mobile population because they’re constantly on the move and are not set in one location like a clumped or uniform population.

Answer:

Mobile population

Explanation: I got it right

Use an examples to distinguish dominant and recessive phenotypes.
I'm a bit stuck and need some help. Much appreciated!

Answers

Think recessive phenotypes as paper and dominant phenotypes as teared paper. Once paper is teared, it can't be fixed. Not even tape. It'll just leave that mess exposed. Same with dominant phenotypes. It just takes one dominant trait to change the looks of future offspring.

If you don't tear the paper, everything is fine. Same with recessive phenotypes. As long as there's no contact with any dominant phenotypes, the looks of future offspring will change.

Visible light comes from which one of the sun's layers?
O A. The radiative zone
B. The corona
C. The convection zone
D. The photosphere
O E. The core

Answers

Answer:

The correct answer is photosphere.

Explanation:

Visible light comes from the layer known as the photosphere.

The Sun's photosphere is the layer that emits visible light. The Sun's atmosphere has seven layers: The core, or the Sun's interior.  The radiative zone. The convective zone. The photosphere or the visible surface. The Chromosphere. And the corona, the outermost layer.

Visible light comes from one of the sun's layers called the photosphere, which is in option D, as the photosphere is the visible surface of the sun and is the layer from which most of the sun's visible light is emitted.

The sun consists of several layers, and each layer is with distinct characteristics. such as the innermost region, have the core, followed by the radiative zone, the convection zone, the photosphere, the chromosphere, and finally, the outermost layer called the corona. In the core, nuclear fusion takes place, and as a result, enormous energy is released.

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What is one condition that must be met for a population to be in genetic equilibrium?

Answers

Answer:

Hardy-Weinberg equilibrium

Explanation:

In order for the population's gene pool frequencies to remain constant, the Hardy-Weinberg equilibrium must be met. Random mating, no mutation, migration, or natural selection must all be present, as well as a big sample size.

OAmalOHopeO

Identify parts A and C.​

Answers

Answer:

A. Liver B. Stomach

Explanation:

Liver detoxifies chemicals and metabolize drugs, stomach mixes the food and grind it

A: Liver

C: Stomach

hope it helps!!!!

Look at the branching tree diagram below.

A slanting, horizontal line is shown. On the extreme left, there is a label that says Common Ancestor. Along the slanting, horizontal line there are five dots labeled from left to right with the following traits Four limbs, Amniotic egg, Fur, Shearing teeth, and Retractable claws. There is one vertical line between each of the consecutive five dots. The lines are labeled from left to right as Amphibians, Reptiles, Marsupials, Dogs, Cats.

Which group of organisms evolved after dogs?

Only cats
Only marsupials
Both cats and marsupials
Both amphibians and reptiles

Answers

Answer:

both cats and marsupials

Answer:   Both Amphibians and Reptiles

Explanation: I took the test and got it, so.....

What are the pigments in cyanobacteria used for?

Answers

Answer:

Pigments are useful to plants and other autotrophs

Explanation:

Not sure, but hope this helped a bit

For the numbered steps below, select the option that places them in the correct order. 1) The ribosome binds to the mRNA and uses tRNAs to translate mRNA into the corresponding amino acid polypeptide sequence. 2) The spliceosome removes introns. 3) The primary structure of the polypeptide chain undergoes hierarchical foldings to form the tertiary structure. 4) RNA polymerase binds to the promoter region of a gene and initiates transcription.

Answers

Answer:

first 4, then 2, then 1, and lastly 3

Explanation:

First, RNA polymerase binds to the promoter region of a gene on the DNA and initiates transcription. Transcription is the process by which we get mRNA (coding RNA) which is used as a template for protein synthesis. Then, in transcription, the resulting mRNA undergoes modification and maturation. This  involves removal of introns, addition of 3'-poly-A tail and 5'-cap. Introns are sequences that do not code for protein and are hence removed. The structure that removes introns is the spliceosome. Thirdly, mature mRNA travels to the cytoplasm. Ribosome (composed of rRNA) binds to mRNA and tRNA to start the process of protein synthesis. The process of formation of protein is called translation. Lastly, the primary structure of protein may undergo folding to form a tertiary structure.

What can young people do about climate change?

Answers

spread the word, avoid plastic, use organic stuff
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Find area and perimeter of shape but perimeter is not 96 hey everyone, What is earthquake mention the effects of earthquake. Determine whether the following individual events are independent or dependent. Then find the probability of the combined event. Randomly drawing and immediately eating two red pieces of candy in a row from a bag that contains red pieces of candy out of pieces of candy total. I need help can sombody tell me the awnser please answer fast............ can you find the answer for m What is a district ? 7. Ten samples of 15 parts each were taken from an ongoing process to establish a p-chart for control. a. Develop a p-Chart for 95 percent confidence (1.96 standard deviation). b. Based on the plotted data points, what comments can you make A population of bacteria contains 80 bacteria in generation 1 and triples inpopulation every generation. This sequence represents the number ofbacteria for the first few generations:80, 240, 720, ...What is the explicit formula for the number of bacteria in generation n?A. a(n) = 80 + (n - 1)3B. a(n) = 3.801-1C. a(n) = 3 + (n-1)80D. a(n) = 80.30-1 Question 12 of 20Which constitutional amendment expanded the right to vote?A. Twenty-SecondB. Twenty-ThirdC. Twenty FifthD. Twenty-FourthSSM Many Plains nations depended upon which animal for their survival? A.horse B.elk C. deer D.buffalo 05) Identify the statement that is true about atoms as they pass through the rock cycle. a. Those that were adjacent to each other remain near each other. b. They do not move through the rock cycle at the same rate. c. They move through the rock cycle in the same manner. d. They stay within the same mineral. A garden is rectangular with a width of 8 feet and a length of 10 feet. If it is surrounded by a walkway 2 feet wide, how many square feet of area does the walkway cover? You and a coworker seem to have a hard time seeing eye - to - eye on a number of different things . Today , she does something that makes you especially angry . What should you do? a) Tell your boss that you can't work with her anymore . b) Talk to your coworkers about how much she is driving you crazy. c) Scream at her that you've had enough of her behavior. d ) Walk away until you can speak with her calmly about the issues you have been having All of the following are TRUE for activities and activity coefficients, except: A) activity for a chemical species is the product of concentration and activity coefficient. B) the activity coefficient corrects for non-ideal behavior due to ionic strength. C) as ionic strength increases, the value of the activity coefficient increases. D) for ions, the activity coefficient approaches unity as the ionic strength approaches 0. E) the activity coefficient for neutral molecules is approximately unity. which one of the following is not true about a rectangle? A. all its angels are right angels B. its opposite angels are equal C.opposite sides are parallel D. all its sides are equal add question tags to the following statements2. please come with me,...?3. he didn't call,...?4. elisha lives near you,...?5. let's go to the park,...?6. you had fun last night,...?7. they've already sent the invitations,...? Read the following excerpt from The Story of My Life by Helen Keller and then answer the question that follows.The morning after my teacher came she led me into her room and gave me a doll. The little blind children at the Perkins Institution had sent it and Laura Bridgman had dressed it; but I did not know this until afterward. When I had played with it a little while, Miss Sullivan slowly spelled into my hand the word "d-o-l-l." I was at once interested in this finger play and tried to imitate it. When I finally succeeded in making the letters correctly I was flushed with childish pleasure and pride. Running downstairs to my mother I held up my hand and made the letters for doll. I did not know that I was spelling a word or even that words existed; I was simply making my fingers go in monkey-like imitation. In the days that followed I learned to spell in this uncomprehending way a great many words, among them pin, hat, cup and a few verbs like sit, stand and walk. But my teacher had been with me several weeks before I understood that everything has a name. I left the well-house eager to learn. Everything had a name, and each name gave birth to a new thought. As we returned to the house every object which I touched seemed to quiver with life. That was because I saw everything with the strange, new sight that had come to me.Which of the following best summarizes the excerpt?A.Helen was excited to learn that everything had a name so that she could talk with her teacher.B.Helen's teacher taught her to finger spell doll so she knew how to tell her teacher what toy she wanted to play with.C.Helen's teacher taught her all objects have a name opening the world of language.D.Helen liked to touch all the objects in the house so she knew which ones she wanted to play with. HELP THANKS SO MUCH BRAIN FOR CORRECT What's a comprehensive electronic or paper-based record of health information controlled by the patient, by which management, access, and sharing of the medical record can occur?